HB Mepụta Paradigm Ọhụrụ maka Nchekwa Sample Biological na ICL

Imperial College London (ICL) na-ebute ụzọ na nyocha sayensị na, site na Ngalaba Immunology na Inflammation na Department of Brain Sciences, nyocha ya sitere na rheumatology na hematology ruo dementia, ọrịa Parkinson na ọrịa kansa ụbụrụ. Ijikwa nyocha dị iche iche dị otú ahụ chọrọ akụrụngwa ọgbara ọhụrụ, ọkachasị maka nchekwa nke ihe nlere anya dị mkpa. Neil Galloway Phillipps, Onye isi nchịkwa ụlọ nyocha maka ngalaba abụọ ahụ, ghọtara mkpa ọ dị maka ngwọta nchekwa cryogenic dị mma na nke na-adigide.

ICL chọrọ

1.Sistemụ nchekwa mmiri nitrogen nwere ikike dị elu

2.Mbelata oriri nitrogen na ọnụ ahịa ọrụ

3.Nchekwa nlele emelitere yana nnabata usoro iwu

4.Ebe nchekwa dị mma yana nke ọma maka ndị nyocha

5.Ihe ngwọta na-adigide iji kwado atụmatụ ndụ ndụ ndụ

Ihe ịma aka

Ngalaba ICL's Immunology dabere na mbụ na nitrogen mmiri mmiri static 13 (LN₂) tankị iji chekwaa ihe nlele ule ụlọ ọgwụ, sel satịlaịtị na omenala sel mbụ. Usoro a kewara ekewa na-ewe oge iji nọgide na-enwe, na-achọ nlekota mgbe niile na njuputa.

Neil kọwara, sị: “Idochi tankị 13 were oge buru ibu, ilekọta ihe niile na-esiwanyekwa ike. "Ọ bụ ihe ịma aka ngwa ngwa, anyị chọkwara ụzọ dị mma karị iji jikwaa nchekwa anyị."

Ọnụ ego nke ijikwa ọtụtụ tankị bụ nchegbu ọzọ. LN₂oriri dị elu, na-atụnye ụtụ na ịrị elu mmefu ọrụ. N'otu oge ahụ, mmetụta gburugburu ebe obibi nke nnyefe nitrogen na-emekarị na-emegide nkwenye ụlọ nyocha ahụ maka nkwado. Neil kwuru, "Anyị na-arụ ọrụ iji nweta ihe nrite nkwado dị iche iche, anyị makwaara na ibelata ojiji nitrogen ga-eme nnukwu mgbanwe."

Nchebe na nrube isi bụkwa ihe kacha mkpa. Ebe ọtụtụ tankị gbasapụrụ n'akụkụ dị iche iche, ịchọta ohere na idebe ihe ndekọ ọhụrụ dị mgbagwoju anya. Neil kwukwara, "Ọ dị mkpa na anyị maara kpọmkwem onye na-enweta ihe nlele ahụ, yana na echekwara ihe niile nke ọma n'usoro iwu Human Tissue Authority (HTA). "Ụdị usoro ochie anyị emeghị ka ọ dị mfe."

Ihe ngwọta

ICL enweelarị ngwa dị iche iche sitere na Haier Biomedical - nchekwa nchekwa oyi, nchekwa nchekwa ndu, CO.₂incubators na centrifuges - ịzụlite ntụkwasị obi na ngwọta nke ụlọ ọrụ.

Ya mere Neil na ndị otu ya gakwuuru Haier Biomedical iji nyere aka dozie nsogbu ọhụrụ ndị a, na-etinye nnukwu ikike CryoBio 43 LN.₂biobank ijikọba tankị 13 static n'ime otu sistemụ arụmọrụ dị elu. Mgbanwe ahụ enweghị nkebi, ya na ndị otu Haier na-ahụ maka nrụnye na ọzụzụ ndị ọrụ ụlọ nyocha. Usoro ọhụrụ ahụ etinyere n'ime LN dị adị₂akụrụngwa nwere naanị obere mgbanwe. Site na usoro ọhụrụ a na-arụ ọrụ, nchekwa ihe nlele na njikwa ejirila nke ọma rụọ ọrụ nke ọma. "Otu n'ime uru anyị na-atụghị anya ya bụ ego ole anyị nwetara," Neil kwuru. "Ebe e wepụrụ tankị ochie ahụ, anyị nwere ọtụtụ ohere na ụlọ nyocha maka akụrụngwa ndị ọzọ."

Ngbanwe na nchekwa vapour-phase emeela ka nchekwa ma dị mfe iji. "N'oge gara aga, oge ọ bụla anyị wepụrụ rack na tank mmiri mmiri, ọ ga-abụ na-asọpụta na nitrogen, bụ nke na-eche nche mgbe niile.

Neil na ndị otu ya chọpụtara na sistemụ ahụ nwere nghọta iji, yana mmemme ọzụzụ Haier na-enyere ha aka ịbanye ngwa ngwa ndị ọrụ njedebe.

Ihe a na-atụghị anya ya ma ọ bụ ihe a na-anabata bụ nzọụkwụ a na-emegharị akpaghị aka, nke na-eme ka ịnweta tankị dị mfe. "Site na tankị ndị gara aga, ndị na-eme nchọpụta na-ebulikarị ihe n'ụzọ zuru ezu. Ọ bụ ezie na tank ọhụrụ ahụ dị ogologo, usoro ndị a na-etinye na ntinye nke bọtịnụ, na-eme ka ịgbakwunye ma ọ bụ wepụ ihe nlele dị mfe ijikwa," Neil kwuru.

Na-echekwa ihe nlele bara uru

Ihe nlele ndị echekwara na akụrụngwa cryogenic nke ICL bara uru maka nyocha na-aga n'ihu. Neil kwuru, "Ụfọdụ n'ime ihe nlele anyị na-echekwa enweghị ike dochie ya kpamkpam.

"Anyị na-ekwu maka nkwadebe mkpụrụ ndụ ọbara ọcha site na ọrịa ndị na-adịghị ahụkebe, ihe nlele ụlọ ọgwụ na ihe ndị ọzọ dị mkpa maka nyocha. A naghị eji ihe nlele ndị a eme ihe n'ime ụlọ nyocha; a na-ekerịta ha na ndị na-arụkọ ọrụ ọnụ gburugburu ụwa, na-eme ka iguzosi ike n'ezi ihe ha dị oke mkpa. Anyị nwere ike ịlele profaịlụ ọnọdụ okpomọkụ n'oge ọ bụla, ma ọ bụrụ na enyochala anyị, anyị nwere ike iji obi ike gosi na echekwala ihe niile nke ọma. "

 Ịkwalite nkwado na arụmọrụ ọnụ ahịa

Iwebata ụlọ akụ ọhụrụ biobank ebelatala oriri nitrogen mmiri mmiri nke ụlọ nyocha ahụ, belata ya okpukpu iri. "Nke ọ bụla n'ime tankị ochie ndị ahụ nwere ihe dị ka lita 125, ya mere ime ka ha sie ike emeela nnukwu ihe," Neil kọwara. "Anyị na-eji ntakịrị nitrogen nke anyị mere na mbụ, na nke ahụ bụ nnukwu mmeri ma n'ụzọ ego na gburugburu ebe obibi."

Site na nnyefe nitrogen dị ole na ole achọrọ, ikuku carbon e belatara, na-akwado ebumnuche nkwado ụlọ nyocha. "Ọ bụghị naanị maka nitrogen n'onwe ya," Neil kwukwara. "Inwe obere nnyefe pụtara obere gwongworo n'okporo ụzọ, yana obere ume eji emepụta nitrogen na mbụ." Nkwalite ndị a dị oke mkpa nke na Imperial nwetara onyinye nkwado site na LEAF na My Green Lab iji kwado mbọ ya.

Mmechi

Haier Biomedical's cryogenic biobank agbanweela ikike nchekwa ICL, na-emeziwanye arụmọrụ, nchekwa na nkwado ebe na-ebelata ọnụ ahịa nke ukwuu. Site na nrubeisi ka mma, nchekwa nlele emelitere yana mbelata mmetụta gburugburu ebe obibi, nkwalite ahụ abụrụla ihe ịga nke ọma.

Nsonaazụ oru ngo

1.LN₂oriri belatara site na 90%, mbelata ọnụ ahịa na ikuku

2.Nlebanya nlele nke ọma na nnabata HTA

3.Nchekwa vapour-phase dị nchebe maka ndị nyocha

4.Enwekwu ike nchekwa na otu sistemụ

5.Nkwenye site na onyinye nkwado nkwado